HaloTag - Wikipedia
WEBHaloTag is a self-labeling protein tag. It is a 297 residue protein (33 kDa) derived from a bacterial enzyme, designed to covalently bind to a synthetic ligand. The bacterial enzyme can be fused to various proteins of interest. [1]
Protein tag - Wikipedia
WEBProtein tags are peptide sequences genetically grafted onto a recombinant protein. Tags are attached to proteins for various purposes. They can be added to either end of the target protein, so they are either C-terminus or N-terminus specific or are both C-terminus and N-terminus specific. Some tags are also inserted at sites within the protein ...
SNAP-tag® Technologies: Tools to Study Protein Function - NEB
WEBNew England Biolabs now introduces a set of protein tools for the specific labeling of fusion proteins with synthetic probes that will help scientists illuminate various aspects of protein function.
HaloTag: A Novel Protein Labeling Technology for Cell Imaging …
WEBJun 6, 2008 · The protein tag (HaloTag) is a modified haloalkane dehalogenase designed to covalently bind to synthetic ligands (HaloTag ligands). The synthetic ligands comprise a chloroalkane linker attached to a variety of useful molecules, such as fluorescent dyes, affinity handles, or solid surfaces.
Visualizing and Manipulating Biological Processes Using …
WEBJul 7, 2020 · Self-labelling protein tags have enabled chemical biology studies in a wide range of biological pathways and cellular models. The versatility of these systems for protein labelling allows chemical biologists to incorporate nearly any functional probe into a …
Chemical Tags for Labeling Proteins Inside Living Cells
WEBAug 31, 2011 · The fluorescent proteins (FPs) revolutionized our ability to study protein function directly in the cell by enabling individual proteins to be selectively labeled through genetic encoding of a fluorescent tag.
Chemical Tags for Labeling Proteins Inside Living Cells
WEBSep 9, 2011 · To overcome the selectivity limitations of a short peptide tag, protein-based chemical tags were introduced that allowed the target protein to be tagged with a protein receptor or enzyme, which can be subsequently labeled with a cell-permeable organic ligand- or substrate-probe heterodimer.
Visualizing and Manipulating Biological Processes by Using …
WEBJul 16, 2020 · Methods of biorthogonal protein labeling are important tools to attain this goal. In this review, we discuss advances in probe technology specific for self-labeling protein tags, focusing mainly on the application of HaloTag and SNAP-tag systems.
Chemical tags and beyond: Live‐cell protein labeling technologies …
WEBIn this review, we focus on tags that can covalently label intracellular proteins and are widely used in biological research. Designing a protein tag is generally inspired by the following patterns: receptor-ligand, enzyme-substrate, and enzyme-inhibitor.
Plasmids 101: Protein tags - Addgene
WEBProtein tags can be used for purification, detection, localization, and more. Learn more about the molecular tool box of tags and fusion proteins.